Icad antibody free#

The expression of the caspase-resistant form of dICAD/DREP-1 in a Drosophila neuronal cell line prevented the apoptotic DNA fragmentation. Biochemical fractionation and immunoprecipitation of dICAD from S2 cell extracts indicated that dICAD is complexed with dCAD in proliferating cells. The recombinant DREP-1/dICAD was cleaved at a specific site by human caspase 3 as well as by extracts prepared from S2 cells undergoing apoptosis. An antibody against DREP-1 inhibited the ICAD activity in the S2 cell extracts, confirming the identification of DREP-1 as a Drosophila homolog of ICAD (dICAD). To identify the inhibitor, we tested recombinant DREP-1, which was previously identified using the Drosophila EST data base and found it also inhibited dCAD DNase. When cell lysates from Drosophila S2 cells were chemically denatured and the denatured proteins were removed after dialysis, the supernatant inhibited Drosophila CAD (dCAD). Two alternatively spliced transcript variants encoding distinct isoforms have been found for the ICAD gene.DNA fragmentation, a hallmark of apoptosis, is regulated by a specific nuclease called caspase-activated DNase (CAD) and its inhibitor (ICAD).

Icad antibody free#

There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells. Immunocytochemistry - Anti-ICAD antibody EPR4220 - BSA and Azide free (ab247711) This data was developed using ab108924, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab108924 at a dilution of 1/250. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45 and a 35kDa isoform, ICAD-S/DFF35. Rabbit anti Murine CAD antibody recognizes a band of 40 kDa in western blotting. CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA. ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. Rabbit anti Murine CAD antibody recognizes murine CAD (Caspase Activated Deoxyribonuclease), the murine homologue of human DFF-40. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released. Spectificity: ICAD Polyclonal Antibody detects endogenous levels of ICAD protein. ICAD Antibody - 100 g - 600-401-BS7 from Rockland Immunochemicals. For long-term storage, aliquot and store product at -20В° C or below, avioiding multiple freeze-thaw cycles. Form: Liquid in PBS containing 50 glycerol, 0.5 BSA and 0.02 sodium azide. ICAD is the inhibitor of caspase-3-activated DNase which is a substrate that controls nuclear apoptosis. For short-term storage (1-2 weeks), product can be stored at 4вЊ. Centrifuge briefly prior to opening the vial. Optimal working dilutions must be determined by end user. Key Applications: Western Blotting Application Notes: Western blot: 1:500-1:1000 Mouse lung tissue lysate can be used as a positive control and a 45 kDa band should be detected. Store product as a concentrated solution. 2-21 of mouse ICAD) is an antibody against ICAD/DFF for use in WB.

The PA5-34818 immunogen is recombit fragment corresponding to a region within amino acids 56 and 316 of ICAD (Uniprot IDNo.O00273). PA5-34818 targets ICAD in WB applications and shows reactivity with Human samples. ICAD antibody ICAD-L antibody ICAD-S antibody zgc:92229 antibody Protein level used designations for DFFA DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, inhibitor of CAD, DFF-45, DNA fragmentation factor, 45kDa, alpha polypeptide.